Getting Prompted for Username and Password on Internal Web Site, HTTP Error 401.2 - Unauthorized.

I have an internal web site that uses Windows Authentication.

This works perfectly on my Microsoft Windows Server 2022 Datacenter Azure Edition which is my developer server.

When I tried to put this site out on our test Microsoft Windows Server 2022 Datacenter Azure Edition, with the exact same setup, I get prompted for my Username and Password. It does not matter what I have tried to enter for Username and Password, nothing is ever accepted and it I hit cancel, I get the dreaded HTTP Error 401.2 - Unauthorized. I don't understand why this would work on 1 server, but not the other server.